S scanning / S-chip

1. Technical Aspects

 

Stem cells are undifferentiated cells that can differentiate into specialized cells and become any tissues in body. Many researches about stem cells are ongoing, to apply stem cell treatments for neurodegenerative diseases and conditions, diabetes, heart diseases, and incurable diseases.

 

Stem cells could be developed as cancer cells during culture. When cells are grown in culture, some cells may lose the normal mechanisms that control cell growth through chromosomal abnormalities. Therefore, stem-cell screening for detecting chromosomal abnormalities is needed before the injection into patient..

 

S-scanning is a service offered by MGMED using DNA-chip for the chromosomal abnormalities in stem cells.

2. Procedure

 

S-chip is a DNA chip designed for detecting chromosomal alterations in stem cells. DNA fragments from frequently altered regions, are attached to a chip.

 

In a clinic stem cell samples are collected and sent to a laboratory.

 

In a laboratory, DNA is extracted from stem cell, labeled with green fluorescent dye, and hybridized. To determine numerical and structural chromosomal abnormalities, normal DNA labelled with red fluorescent dye is competitively hybridized as control.